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DEVELOPMENT AND OPTIMIZATION OF PACKING CHROMATOGRAPHIC COLUMNS
Černá, Kateřina ; Kozlík, Petr (advisor) ; Kubíčková, Anna (referee)
The aim of this thesis was to develop and optimize the packing of a new series of chromatographic columns called Astra with subsequent analytical measurements to determine the quality of optimized packing processes, the quality of the columns and to investigate the behavior of stationary phases. The packing processes for the following columns were developed and optimized - Astra C18 HE, AQ and DM 5 µm (250/150/100×4,6 mm, 150/100×3,0 mm), Astra C18-HE 3 µm (250/150/100×4,6 mm) , 150/100/50×3,0 mm, 150/100/75/50×2,1 mm), Astra AQ 3 µm (150/100×4,6 mm, 150/100/50x3,0 mm, 150/100/75/50×2,1 mm), Astra DM 3µm (150/100×4,6 mm, 150/100x3,0 mm, 150/100/50×2,1 mm) and for zwitterionic columns 3µm (150×4,6 mm, 100×2,1 mm). During the development of the packing processes, the following packing parameters were optimized. Composition of the suspension solution (slurry) - cyclohexanol:chloroform (1:10) for SF C18, AQ, DM and glycerin:methanol (1:3) for zwitterionic SF; concentration of the packing suspension; column pre-filling method - pre-filling with packing liquid (methanol); PID parameters, the values P = 3000, I = 100 and D = 100 were chosen; width of the pre-column, which must be equal to or greater than the inside diameter of the column; packing pressure and time; the column hardware type was chosen...
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